PVRL1 Protein (Fc Tag)
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- Antigène Voir toutes PVRL1 Protéines
- PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette PVRL1 protéine est marqué à la Fc Tag.
- Fonction
- Recombinant Human CD111/Nectin-1 Protein (Fc Tag)(Active)
- Séquence
- Met 1-Thr 334
- Attributs du produit
- A DNA sequence encoding the human PVRL1 isoform 1 (NP_002846.3) extracellular domain (Met 1-Thr 334) was was fused with the Fc region of human IgG1 at the C-terminus.
- Pureté
- > 98 % as determined by reducing SDS-PAGE.
- niveau d'endotoxine
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized rh PVRL3 at 1 μg/ml (100 μl/well) can bind recombinant human Nectin-1 with a linear range of 0.156-5 ng/ml.
- Top Product
- Discover our top product PVRL1 Protéine
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Antigène
- PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))
- Autre désignation
- CD111/Nectin-1 (PVRL1 Produits)
- Synonymes
- CD111 Protein, CLPED1 Protein, ED4 Protein, HIgR Protein, HVEC Protein, OFC7 Protein, PRR Protein, PRR1 Protein, PVRR Protein, PVRR1 Protein, SK-12 Protein, nectin-1 Protein, prr1 Protein, PVRL1 Protein, pvrl1 Protein, AI835281 Protein, AW549174 Protein, Cd111 Protein, HveC Protein, cd111 Protein, cd112 Protein, clped1 Protein, ed4 Protein, higr Protein, hveb Protein, hvec Protein, nectin-2 Protein, ofc7 Protein, prr Protein, prr2 Protein, pvrl2 Protein, pvrl2.L Protein, pvrr Protein, pvrr1 Protein, pvrr2 Protein, sk-12 Protein, nectin cell adhesion molecule 1 Protein, nectin-1 Protein, Poliovirus receptor-related protein 1 Protein, poliovirus receptor-related 1 (herpesvirus entry mediator C) Protein, nectin cell adhesion molecule 2 L homeolog Protein, NECTIN1 Protein, LOC100145553 Protein, pvrl1 Protein, nectin1 Protein, Nectin1 Protein, PVRL1 Protein, nectin2.L Protein
- Sujet
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Background: Poliovirus receptor-related 1 (herpesvirus entry mediator C, nectin-1, CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV). CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.
Synonym: Poliovirus Receptor-Related Protein 1, Herpes Virus Entry Mediator C, Herpesvirus Entry Mediator C, HveC, Herpesvirus Ig-Like Receptor, HIgR, Nectin-1, CD111, PVRL1, HVEC, PRR1,ED4,HIgR,HV1S,HVEC,nectin-1,OFC7,PRR,PVRR,PVRR1,SK-12
- Poids moléculaire
- 61 kDa
- NCBI Accession
- NP_002846
- Pathways
- Cell-Cell Junction Organization
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