RAC1 Protein (GST tag)

N° du produit ABIN7197633

Détail du produit

Antigène: RAC1

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette RAC1 protéine est marqué à la GST tag.

Fonction: Recombinant Human RAC1/MIG5 Protein (GST Tag)

Séquence: Met 1-Cys 189

Attributs du produit: A DNA sequence encoding the mature form of human Rac1 isoform A (NP_008839.2) (Met 1-Cys 189) was expressed with the GST tag at the N-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: RAC1

Autre désignation: RAC1/MIG5 (RAC1 Produits)

Synonymes: Rac-1 Protein, TC-25 Protein, p21-Rac1 Protein, AL023026 Protein, D5Ertd559e Protein, RAC2 Protein, CRAC1A Protein, rac1 Protein, wu:fd16e02 Protein, zgc:55823 Protein, zgc:55917 Protein, zgc:86934 Protein, 2248 Protein, CG2248 Protein, D-Rac Protein, D-Rac 1 Protein, D-Rac1 Protein, DRac Protein, DRac1 Protein, DRacA Protein, Dm Rac1 Protein, DmRAC Protein, DmRAC1 Protein, Dmel\CG2248 Protein, Dmrac1 Protein, Drac Protein, Drac1 Protein, Drac1a Protein, DracA Protein, GTPase Rac1 Protein, P40792 Protein, RAC1 Protein, Rac Protein, Rac GTPase Protein, Rac3 Protein, RacA Protein, dRac Protein, dRac1 Protein, drac1 Protein, rac Protein, Xrac Protein, mig5 Protein, p21-rac1 Protein, tc-25 Protein, Rac family small GTPase 1 Protein, RAS-related C3 botulinum substrate 1 Protein, ras-related C3 botulinum toxin substrate 1 Protein, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) Protein, Rac family small GTPase 1a Protein, CG2248 gene product from transcript CG2248-RB Protein, Rac family small GTPase 1 L homeolog Protein, ras homolog family member G Protein, RAC1 Protein, Rac1 Protein, rac1a Protein, rac1.L Protein, RHOG Protein

Sujet:

Background: RAC1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for RAC1 gene. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. RAC1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophage. RAC1 is essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. RAC1's isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. Stat3 is an important transcription factor that regulates both proinflammatory and anit-apoptotic pathways in the heart. It forms a multiprotein complex with RAC1 and PKC in an H/R-dependent manner by expression of constitutively active Rac1 mutant protein, and by RNA silencing of RAC1. Selective inhibition of PKC with calphostin C produces a marked suppression of Stat3 S727 phosphorylation. The association of Stat3 with Rax1 occurs predominantly at the cell membrane, but also inside the nucleus, and occurs through the binding of the coiled-coil domain of Stat3 to the 54 NH(2)-terminal residues of RAC1. Transfection with a peptide comprising the NH(2)-terminal 17 amino acid residues of RAC1-dependent signaling pathways resulting in physical association between Rac1 and Stat3 and the formation of a novel multiprotein complex with PKC.

Synonym: MIG5,p21-Rac1,Rac-1,TC-25

Poids moléculaire: 47 kDa

NCBI Accession: NP_008839

Pathways: Signalisation WNT, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling