USP5 Protein (His tag)
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- Antigène Voir toutes USP5 Protéines
- USP5 (Ubiquitin Specific Peptidase 5 (USP5))
- Type de proteíne
- Recombinant
- Origine
- Humain
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Source
- Baculovirus infected Insect Cells
- Purification/Conjugué
- Cette USP5 protéine est marqué à la His tag.
- Fonction
- Recombinant Human USP5/ISOT Protein (His Tag)
- Séquence
- Met 1-Ser 835
- Attributs du produit
- A DNA sequence encoding the human USP5 isoform short (P45974-2) (Met 1-Ser 835) was fused with a polyhistidine tag at the C-terminus.
- Pureté
- > 92 % as determined by reducing SDS-PAGE.
- niveau d'endotoxine
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product USP5 Protéine
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 7.4
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Antigène
- USP5 (Ubiquitin Specific Peptidase 5 (USP5))
- Autre désignation
- USP5/ISOT (USP5 Produits)
- Synonymes
- ISOT Protein, AA407472 Protein, ISOT-1 Protein, Ucht Protein, ubiquitin specific peptidase 5 Protein, ubiquitin carboxyl-terminal hydrolase 5 Protein, ubiquitin carboxyl-terminal hydrolase 5, putative Protein, ubiquitin specific protease 5 Protein, ubiquitin specific peptidase 5 (isopeptidase T) Protein, USP5 Protein, CNH02890 Protein, EHI_049940 Protein, CC1G_12961 Protein, MCYG_01862 Protein, CGB_L3600C Protein, PY00546 Protein, Usp5 Protein
- Sujet
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Background: Ubiquitin carboxyl-terminal hydrolase 5, also known as Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thiolesterase 5, Ubiquitin-specific-processing protease 5, ISOT and USP5, is a member of the peptidase C19 family. USP5 contains 2 UBA domains and one UBP-type zinc finger. The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition. The UBP-type zinc finger domain crystallizes as a dimer linked by a disulfide bond between the Cys-195 residues of both molecules, but there is no evidence that the full-length USP5 exists as a dimer. USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. USP5 is involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. It binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
Synonym: ISOT
- Poids moléculaire
- 94.7 kDa
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