UBE2F Protein (His tag)
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- Antigène Voir toutes UBE2F Protéines
- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
- Type de proteíne
- Recombinant
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette UBE2F protéine est marqué à la His tag.
- Fonction
- Recombinant Human UBE2F Protein (His Tag)
- Séquence
- Met 1-Arg 185
- Attributs du produit
- A DNA sequence encoding the human UBE2F isoform 1 (Q969M7-1) (Met 1-Arg 185) was expressed, with a polyhistide tag at the N-terminus.
- Pureté
- > 94 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product UBE2F Protéine
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, 10 % glycerol, 2 mM DTT, pH 7.5
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Antigène
- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
- Autre désignation
- UBE2F (UBE2F Produits)
- Synonymes
- NCE2 Protein, 2510010F15Rik Protein, AI851109 Protein, RGD1307608 Protein, zgc:77005 Protein, ubiquitin conjugating enzyme E2 F (putative) Protein, NEDD8-conjugating enzyme UBE2F Protein, ubiquitin-conjugating enzyme E2F (putative) Protein, ubiquitin conjugating enzyme E2F (putative) S homeolog Protein, UBE2F Protein, Tsp_01616 Protein, ube2f Protein, ube2f.S Protein, Ube2f Protein
- Sujet
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Background: UBE2F is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.
Synonym: NCE2
- Poids moléculaire
- 22.9 kDa
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