VCAM1 Protein (His tag)
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- Antigène Voir toutes VCAM1 Protéines
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
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Origine
- Souris
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette VCAM1 protéine est marqué à la His tag.
- Fonction
- Recombinant Mouse VCAM1 Protein (His Tag)(Active)
- Séquence
- Met 1-Glu 698
- Attributs du produit
- A DNA sequence encoding the extracellular domain (Met 1-Glu 698) of mouse VCAM1 (NP_035823.3) was fused with the a polyhistidine tag at the C-terminus.
- Pureté
- > 97 % as determined by SDS-PAGE
- niveau d'endotoxine
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to mouse VCAM1 coated plates (10 μg/ml with 100 μl/well), approximately 70%-80% cells will adhere after 1 hour at RT.
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- Discover our top product VCAM1 Protéine
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Antigène
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
- Autre désignation
- VCAM1 (VCAM1 Produits)
- Synonymes
- CD106 Protein, INCAM-100 Protein, Vcam-1 Protein, VCAM1B Protein, fi76d06 Protein, wu:fi76d06 Protein, zgc:158875 Protein, VCAM1 Protein, vascular cell adhesion molecule 1 Protein, vascular cell adhesion molecule 1b Protein, VCAM1 Protein, Vcam1 Protein, vcam1b Protein, VCAM-1 Protein
- Sujet
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Background: Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Synonym: CD106;Vascular cell adhesion protein 1; Vcam1; L1CAM;VCMA1;Vcam-1
- Poids moléculaire
- 75.8 kDa
- NCBI Accession
- NP_035823
- Pathways
- Carbohydrate Homeostasis
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