IDH1 Protein (Arg132Cys-Mutant) (His tag)

N° du produit ABIN7273421

Détail du produit

Antigène: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Type de proteíne: Recombinant

Attributs du protein: Arg132Cys-Mutant

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette IDH1 protéine est marqué à la His tag.

Fonction: Human IDH1 (R132C) Protein, His Tag (MALS verified)

Séquence: Met 1 - Leu 414

Attributs du produit: Human IDH1 (R132C), His Tag (ID1-H51H8) is expressed from E.coli cells. It contains AA Met 1 - Leu 414 (Accession # O75874-1(R132C).

Pureté: >95 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Classe de qualité: MALS verified

Information d'application

Indications d'application: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 48.5 kDa. The protein migrates as 47-50 kDa under reducing (R) condition due to glycosylation.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH 7.5

Stock: -80 °C

Stockage commentaire: -70°C

Détail du antigène

Antigène: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Autre désignation: IDH1 (IDH1 Produits)

Synonymes: IDCD Protein, IDH Protein, IDP Protein, IDPC Protein, PICD Protein, NADP-CICDH Protein, AI314845 Protein, AI788952 Protein, E030024J03Rik Protein, Id-1 Protein, Idh-1 Protein, Idpc Protein, cb876 Protein, fm90e09 Protein, im:7143416 Protein, wu:fm90e09 Protein, F23E12.180 Protein, F23E12_180 Protein, IDH-I Protein, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 1 Protein, isocitrate dehydrogenase 1 Protein, isocitrate dehydrogenase I Protein, isocitrate dehydrogenase (NADP(+)) 1, cytosolic Protein, isocitrate dehydrogenase 1 (NADP+), soluble Protein, isocitrate dehydrogenase 1 (NADP+) L homeolog Protein, isocitrate dehydrogenase 1 Protein, IDH1 Protein, Idh1 Protein, idh1.L Protein, idh1 Protein

Sujet: Synonyms:IDH1,PICD,IDP,Description:The IDH enzymes catalyze the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (α-KG), producing nicotinamide adenine dinucleotide phosphate (NADPH) in the process via the citric acid cycle. Eukaryotic cells express two distinct classes of IDHs that utilize either NAD or NADP as their cofactors and serve diverse biological functions. NAD-dependent IDH is localized to the mitochondrial matrix and is well known for its central role for energy production in the Krebs cycle. NADP-dependent IDHs are primarily located either in mitochondria or cytoplasm . Each NADP-dependent isozyme is a homodimer. Mutations of Arg132 of human IDH1 result in a reduced ability of the enzyme to convert isocitrate to alpha -ketoglutarate, but the enzyme acquires the ability to generate 2-hydroxyglutarate (2HG) from alpha-ketoglutarate,2-HG is elevated in several tumor types, including a subset of AMLs.

Poids moléculaire: 48.5 kDa

Pathways: L'effet Warburg