Cette ACE2 protéine est marqué à la Fc-Avi Tag,Biotin.
Fonction
Biotinylated Human ACE2/ACEH Protein
Séquence
Gln18-Ser740
Attributs du produit
Recombinant Biotinylated Human ACE2/ACEH Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Gln18-Ser740.
Pureté
> 95 % as determined by Tris-Bis PAGE
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized SARS-COV-2 Spike S, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ACE2, hFc Tag with the EC50 of 0.63μg/ml determined by ELISA. See testing image for detail.
ACE2
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
ELISA
ACE2
Origine: Macaque rhésus
Hôte: Human Cells
Recombinant
>95 % as determined by reducing SDS-PAGE.
Active
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Sujet
ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.
Poids moléculaire
111.2 kDa. Due to glycosylation, the protein migrates to 115-125 kDa based on Tris-Bis PAGE result.