Recombinant Human ALCAM/CD166 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp28-Ala526.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human ALCAM, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.7μg/ml determined by ELISA. See testing image for detail.
ALCAM
Origine: Rat
Hôte: HEK-293 Cells
Recombinant
> 95 % as determined by SDS-PAGE
Active
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Brain metastasis (BM) in non-small-cell lung cancer (NSCLC) has a very poor prognosis. Recent studies have demonstrated the importance of cell adhesion molecules in tumor metastasis.Elevated levels of ALCAM expression promote BM formation in NSCLC through increased tumor cell dissemination and interaction with the brain endothelial cells. Therefore, ALCAM could be targeted to reduce the occurrence of BM.
Poids moléculaire
57 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.