APOH
(Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))
Type de proteíne
Recombinant
Attributs du protein
AA 20-345
Origine
Cynomolgus
Source
HEK-293 Cells
Purification/Conjugué
Cette APOH protéine est marqué à la His tag.
Fonction
Cynomolgus APOH Protein
Séquence
Gly20-Cys345
Attributs du produit
Recombinant Cynomolgus APOH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Cys345.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus APOH, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 97.2ng/ml determined by ELISA. See testing image for detail.
APOH
Origine: Humain
Hôte: Mammalian Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
APOH
Origine: Rat
Hôte: Escherichia coli (E. coli)
Recombinant
> 95 %
SDS, WB, Imm, PC
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS, 100 mM Arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
APOH
(Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))
Apolipoprotein (apo)H (also known as beta 2 glycoprotein-I) is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. APOH displays a genetically determined structural polymorphism: three alleles (APOH*1, APOH*2, APOH*3) at a single locus on chromosome 17 code for different isoforms, and population studies have shown that APOH*2 is the most frequent allele.
Poids moléculaire
37.39 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.