Cette ApoE3 protéine est marqué à la His tag,Biotin.
Fonction
Biotinylated Human APOE3/Apolipoprotein E Protein (Primary Amine Labeling)
Séquence
Lys19-His317
Attributs du produit
Recombinant Biotinylated Human APOE3/Apolipoprotein E Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus.It contains Lys19-His317.
Pureté
> 95 % as determined by Tris-Bis PAGE
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
The affinity constant of 1.41 μM as determined in SPR assay (Biacore T200). See testing image for detail.
MALS verified
ApoE3
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
AD2 Protein, LDLCQ5 Protein, LPG Protein, apolipoprotein E Protein, APOE Protein
Sujet
Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD).
Poids moléculaire
35.3 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.