Cette beta-2 Microglobulin protéine est marqué à la His tag.
Fonction
Cynomolgus/Rhesus macaque B2M/beta 2-Microglobulin Protein
Séquence
Ile21-Met119
Attributs du produit
Recombinant Cynomolgus/Rhesus macaque B2M/beta 2-Microglobulin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile21-Met119.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
B2M
Origine: Souris
Hôte: Escherichia coli (E. coli)
Recombinant
> 97 %
WB, SDS, Imm, PC
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
The genetic and functional analysis of β2-microglobulin (B2M), a component of the HLA class-I complex.Acquired homozygous loss of B2M that caused lack of cell-surface HLA Class I expression in the tumor and a matched patient-derived xenograft (PDX). Downregulation of B2M was also found in two additional PDXs established from ICI-resistant tumors.
Poids moléculaire
12.7 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Tris-Bis PAGE result.