BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
Type de proteíne
Recombinant
Attributs du protein
AA 134-285
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette BAFF protéine est marqué à la Fc Tag.
Fonction
Human BAFF/TNFSF13B/CD257 Protein
Séquence
Ala134-Leu285
Attributs du produit
Recombinant Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala134-Leu285.
Pureté
> 90 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human BCMA Trimer, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 17ng/ml determined by ELISA. The affinity constant of 5.83 μM as determined in SPR assay (Biacore T200). See testing image for detail.
TNFSF13B
Origine: Humain
Hôte: Tobacco (Nicotiana benthamiana)
Recombinant
> 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
WB, CC
Active
TNFSF13B
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
Poids moléculaire
41.7 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.