BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
Type de proteíne
Recombinant
Attributs du protein
Trimer
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette BAFF protéine est marqué à la His-Avi Tag,Biotin.
Séquence
Thr141-Leu285
Pureté
> 95% as determined by Tris-Bis PAGE,> 94% as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human BAFFR, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human BAFF (Trimer) , His Tag with the EC50 of 6.9ng/ml determined by ELISA. See testing image for detail.
TNFSF13B
Origine: Humain
Hôte: Tobacco (Nicotiana benthamiana)
Recombinant
> 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
WB, CC
Active
TNFSF13B
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Stock
4 °C,-80 °C
Stockage commentaire
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Date de péremption
12 months
Antigène
BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
BAFF, BLyS, CD257, TNFSF13B, TNFSF20, DTL, TALL1, TALL-1delta BAFF, TALL1Delta4 BAFF, THANK, ZTNF4, TALL-1,B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
Poids moléculaire
54.2 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Tris-Bis PAGE result.