Cette EpCAM protéine est marqué à la His-Avi Tag,Biotin.
Fonction
Biotinylated Human EpCAM/TROP1 Protein
Séquence
Gln24-Lys265
Attributs du produit
Recombinant Biotinylated Human EpCAM/TROP1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Lys265.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Biotinylated Human EpCAM, His Tag at 0.2μg/ml (100ul/Well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA. See testing image for detail.
EPCAM
Origine: Rat
Hôte: HEK-293 Cells
Recombinant
> 97 % as determined by SDS-PAGE
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF-like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
Poids moléculaire
30.1 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.