CD2 Protein (CD2) (AA 25-209) (His tag)

N° du produit ABIN7274105

Protéine Recombinant CD2 exprimée dans HEK-293 Cells.

Aperçu rapide pour CD2 Protein (CD2) (AA 25-209) (His tag) (ABIN7274105)

Antigène: CD2

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 25-209

Purification/Conjugué: Cette CD2 protéine est marqué à la His tag.

Fonction: Human CD2/SRBC Protein

Séquence: Lys25-Asp209

Attributs du produit: Recombinant Human CD2/SRBC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys25-Asp209.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human CD2, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD58, hFc Tag with an affinity constant of 15.48 nM as determined in SPR assay (Biacore T200).,Immobilized Human CD2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD58, hFc Tag with the EC50 of 0.32ug/ml determined by ELISA (QC Test).,Human CD2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CD2 is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD2

Autre désignation: CD2

Sujet: The CD2 family of receptors is evolutionarily conserved and widely expressed on cells within the hematopoietic compartment. In recent years several new members have been identified with important roles in the immune system. CD2 family members regulate natural killer (NK) cell lytic activity and inflammatory cytokine production when engaged by ligands on tumor cells.

Poids moléculaire: 22.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

UniProt: P06729