CD27 Protein (AA 21-192) (hFc Tag)

N° du produit ABIN7274113

Cette protéine Recombinant CD27 est exprimée dans HEK-293 Cells.

Aperçu rapide pour CD27 Protein (AA 21-192) (hFc Tag) (ABIN7274113)

Antigène: CD27 (CD27 Molecule (CD27))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 21-192

Purification/Conjugué: Cette CD27 protéine est marqué à la hFc Tag.

Fonction: Human CD27/TNFRSF7 Protein

Séquence: Thr21-Ile192

Attributs du produit: Recombinant Human CD27/TNFRSF7 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Thr21-Ile192.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD27 Ligand Trimer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 of 30.1ng/ml determined by ELISA (QC Test).,Human CD27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CD27 is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD27 (CD27 Molecule (CD27))

Autre désignation: CD27

Sujet: CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co-stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.

Poids moléculaire: 46.2 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.

UniProt: P26842

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints