Recombinant Human CD200 R1/CRTR2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala27-Leu266.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human CD200, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD200 R1, hFc Tag with the EC50 18ng/ml determined by ELISA. See testing image for detail.
CD200R1
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
>95 % as determined by reduced SDS-PAGE.
Active
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand.
Poids moléculaire
53.7 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.