CD28 Protein (CD28) (AA 19-152) (Biotin,His-Avi Tag)

N° du produit ABIN7274256

Cette protéine Recombinant CD28 est produite dans HEK-293 Cells.

Aperçu rapide pour CD28 Protein (CD28) (AA 19-152) (Biotin,His-Avi Tag) (ABIN7274256)

Antigène: CD28

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain, Cynomolgus, Macaque rhésus

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE

Détail du produit

Attributs du protein: AA 19-152

Purification/Conjugué: Cette CD28 protéine est marqué à la Biotin,His-Avi Tag.

Fonction: Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein

Séquence: Asn19-Pro152

Attributs du produit: Recombinant Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asn19-Pro152.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 41.59 uM as determined in SPR assay (Biacore T200).,Immobilized Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA.,Biotinylated Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD28

Autre désignation: CD28

Sujet: CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Poids moléculaire: 18.2 kDa. Due to glycosylation, the protein migrates to 38-55 kDa based on Bis-Tris PAGE result.

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response