FCGR3A
(Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))
Type de proteíne
Recombinant
Attributs du protein
AA 17-208
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette FCGR3A protéine est marqué à la His-Avi Tag.
Fonction
Human Fc gamma RIIIA/CD16a (F176) Protein
Séquence
Gly17-Gln208 (F176)
Attributs du produit
Recombinant Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus..It contains Gly17-Gln208(F176).
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human Fc gamma RIIIA (F176) , His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 85.6ng/ml determined by ELISA. The affinity constant of 0.92μM as determined in SPR assay (Biacore T200). See testing image for detail.
FCGR3A
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
FCGR3A
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
> 90 % as determined by reducing SDS-PAGE.
Active
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
FCGR3A
(Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))
CD16 Protein, CD16A Protein, FCG3 Protein, FCGR3 Protein, FCGRIII Protein, FCR-10 Protein, FCRIII Protein, FCRIIIA Protein, IGFR3 Protein, Fcgr4 Protein, Fc fragment of IgG receptor IIIa Protein, FCGR3A Protein, Fcgr3a Protein
Sujet
Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Poids moléculaire
24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.