CD3 epsilon Protein (CD3E) (Fc Tag)
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- Antigène Voir toutes CD3 epsilon (CD3E) Protéines
- CD3 epsilon (CD3E)
- Type de proteíne
- Recombinant
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Origine
- Cynomolgus
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette CD3 epsilon protéine est marqué à la Fc Tag.
- Séquence
- Gln22-Asp117
- Pureté
- > 95% as determined by Tris-Bis PAGE
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus CD3E, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3E Antibody, mFc Tag with the EC50 of 39.4ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- CD3 epsilon (CD3E)
- Autre désignation
- CD3E (CD3E Produits)
- Synonymes
- cd3e Protein, CD3E Protein, t3e Protein, tcre Protein, AI504783 Protein, CD3 Protein, CD3epsilon Protein, T3e Protein, T3E Protein, TCRE Protein, CD3e molecule Protein, CD3 antigen, epsilon polypeptide Protein, cd3e Protein, CD3E Protein, Cd3e Protein
- Sujet
- CD3e, CD3E, T3E, FLJ18683, TCRE, CD3-epsilon, IMD18,CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
- Poids moléculaire
- 36.9 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Tris-Bis PAGE result.
- Pathways
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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