CLEC12A
(C-Type Lectin Domain Family 12, Member A (CLEC12A))
Type de proteíne
Recombinant
Attributs du protein
AA 32-232
Origine
Cynomolgus
Source
HEK-293 Cells
Purification/Conjugué
Cette CLEC12A protéine est marqué à la His tag.
Fonction
Cynomolgus CLEC12A/MICL/CLL-1 Protein
Séquence
His32-Ala232
Attributs du produit
Recombinant Cynomolgus CLEC12A/MICL/CLL-1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains His32-Ala232.
Pureté
> 95 % as determined by Tris-Bis PAGE
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Anti-CLEC12A Antibody captured on CM5 Chip via Protein A can bind Cynomolgus CLEC12A, His Tag with an affinity constant of 1.28 μM as determined in SPR assay (Biacore T200). See testing image for detail.
CLEC12A
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
CLEC12A
(C-Type Lectin Domain Family 12, Member A (CLEC12A))
MGC179455 Protein, CLL-1 Protein, CLL1 Protein, DCAL-2 Protein, MICL Protein, D230024O04 Protein, KLRL1 Protein, Micl Protein, C-type lectin domain family 12 member A Protein, C-type lectin domain family 12, member a Protein, C-type lectin domain family 12, member A Protein, CLEC12A Protein, Clec12a Protein
Sujet
CLEC12A has recently been identified as an antigen, expressed on leukemic stem cells and leukemic blasts. Given the fact that this expression profile seems stable throughout diagnosis, treatment and relapse on leukemic blasts and leukemic stem cells, CLEC12A can be considered a highly potent and reliable marker for the detection of measurable residual disease and therefore applicable for risk stratification and prognostication in AML.
Poids moléculaire
24.6 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result.