SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 319-541, RBD) (hFc Tag)

N° du produit ABIN7274395

Cette protéine Recombinant SARS-CoV-2 Spike est exprimée dans HEK-293 Cells.

Aperçu rapide pour SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 319-541, RBD) (hFc Tag) (ABIN7274395)

Antigène: SARS-CoV-2 Spike (SARS-CoV-2 S)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 319-541, RBD

Purification/Conjugué: Cette SARS-CoV-2 Spike protéine est marqué à la hFc Tag.

Fonction: SARS-COV-2 Spike RBD Protein

Séquence: Arg319-Phe541

Attributs du produit: Recombinant SARS-COV-2 Spike RBD Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Arg319-Phe541.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus ACE2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.,SARS-COV-2 Spike RBD on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of SARS-COV-2 Spike RBD is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: SARS-CoV-2 Spike (SARS-CoV-2 S)

Autre désignation: SARS-COV-2 Spike

Classe de substances: Viral Protein

Sujet: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Poids moléculaire: 50.7 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.