SARS-CoV-2 Spike Protein (RBD) (His-Avi Tag)

N° du produit ABIN7274399

Détail du produit

Antigène: SARS-CoV-2 Spike

Type de proteíne: Recombinant

Attributs du protein: RBD

Origine: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purification/Conjugué: Cette SARS-CoV-2 Spike protéine est marqué à la His-Avi Tag.

Séquence: Arg319-Asn532

Pureté: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized SARS-COV-2 Spike RBD, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.5ng/ml determined by ELISA. The affinity constant of 13.8nM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: 4 °C,-80 °C

Stockage commentaire: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: SARS-CoV-2 Spike

Abstract: SARS-CoV-2 Spike Produits

Sujet: S protein RBD, S glycoprotein RBD, Spike protein RBD,The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Poids moléculaire: 27 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Tris-Bis PAGE result.