M-CSF/CSF1 Protein (AA 33-190)

N° du produit ABIN7274423

Détail du produit

Antigène: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Type de proteíne: Recombinant

Attributs du protein: AA 33-190

Origine: Humain

Source: HEK-293 Cells

Fonction: Human M-CSF/CSF-1 Protein

Séquence: Glu33-Asn190

Attributs du produit: Recombinant Human M-CSF/CSF-1 Protein is expressed from HEK293 without tag.It contains Glu33-Asn190.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human M-CSF at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 29.6ng/ml determined by ELISA. The affinity constant of 2.06 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Autre désignation: M-CSF (CSF1 Produits)

Synonymes: CSF-1 Protein, MCSF Protein, C87615 Protein, Csfm Protein, op Protein, csf1-1 Protein, zgc:172186 Protein, CSF1 Protein, csf1-2 Protein, zgc:158436 Protein, colony stimulating factor 1 Protein, colony stimulating factor 1 (macrophage) Protein, colony stimulating factor 1a (macrophage) Protein, macrophage colony-stimulating factor 1 Protein, macrophage colony stimulating factor Protein, colony stimulating factor 1b (macrophage) Protein, CSF1 Protein, Csf1 Protein, csf1a Protein, LOC396599 Protein, LOC100860895 Protein, csf1b Protein

Sujet: CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.

Poids moléculaire: 20.5 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Tris-Bis PAGE result.

Pathways: Signalisation RTK