MPXV E8L (AA 1-275) protein (His tag)
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- Antigène
- MPXV E8L
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-275
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Origine
- Mpox Virus
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- His tag
- Fonction
- Monkeypox virus E8L Protein
- Séquence
- Met1-Thr275
- Attributs du produit
- Recombinant Monkeypox virus E8L Protein is expressed from E.coli with His tag at the N-Terminus.It contains Met1-Thr275.
- Pureté
- > 95 % as determined by Tris-Bis PAGE
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- MPXV E8L
- Autre désignation
- E8L
- Sujet
- E8L is an important protein that mediates the invasion of monkeypox virus into host cells. In the process of invading and completing replication, E8L acts as a surface-binding protein of mature virion and can bind to chondroitin sulfate on the cell surface, so that the virus can attach to target cells.
- Poids moléculaire
- 32.92 kDa. The protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
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