Cette EGFR protéine est marqué à la His-Avi Tag,Biotin.
Séquence
Leu25-Ser645
Pureté
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human EGF, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human EGFR, His Tag with the EC50 of 6.4μg/ml determined by ELISA. See testing image for detail.
EGFR
Origine: Cynomolgus
Hôte: Mammalian Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
EGFR
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
Greater than 95 % by SDS-PAGE gel analyses
WB, ELISA
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Stock
4 °C,-80 °C
Stockage commentaire
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
ErbB, EC 2.7.10, EC 2.7.10.1, EGFR, mENA, LEGFR, ERBB, ERBB1, HER1, PIG61, NISBD2,The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Poids moléculaire
71.5 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.