Fc epsilon RI/FCER1A (FCER1A)
(Fc Fragment of IgE Receptor Ia (FCER1A))
Type de proteíne
Recombinant
Attributs du protein
AA 26-205
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette Fc epsilon RI/FCER1A protéine est marqué à la His tag.
Fonction
Human Fc epsilon RI alpha/FCER1a Protein
Séquence
Val26-Gln205
Attributs du produit
Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val26-Gln205.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human Fc epsilon RI alpha, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. See testing image for detail.
SPR verified
FCER1A
Origine: Cynomolgus
Hôte: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
Fc epsilon RI/FCER1A (FCER1A)
(Fc Fragment of IgE Receptor Ia (FCER1A))
FCER1A Protein, FCE1A Protein, FcERI Protein, Iger01 Protein, RATIGER01 Protein, Fce1a Protein, Fcr-5 Protein, fcepsilonri Protein, Fc fragment of IgE receptor Ia Protein, Fc receptor, IgE, high affinity I, alpha polypeptide Protein, FCER1A Protein, Fcer1a Protein
Sujet
Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels.
Poids moléculaire
22.1 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result.