FGFR2 beta (AA 152-378) protein (His-Avi Tag,Biotin)

N° du produit ABIN7274651

Détail du produit

Antigène: FGFR2 beta

Type de proteíne: Recombinant

Attributs du protein: AA 152-378

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: His-Avi Tag,Biotin

Fonction: Biotinylated Human FGFR2 beta (IIIb) Protein

Séquence: Arg152-Glu378

Attributs du produit: Recombinant Biotinylated Human FGFR2 beta (IIIb) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg152-Glu378.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-FGFR2 IIIb Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGFR2 beta (IIIb) , His Tag with the EC50 of 13.1ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: FGFR2 beta

Sujet: Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha  isoform, or only IgII and IgIII, referred to as the beta  isoform.

Poids moléculaire: 28.3 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.