Recombinant Biotinylated Human FGFR2 beta (IIIc) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg152-Glu377.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
FGFR2 beta
Sujet
Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform.
Poids moléculaire
28.2 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.