TNFSF18
(Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))
Type de proteíne
Recombinant
Attributs du protein
Trimer
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette TNFSF18 protéine est marqué à la His-DYKDDDDK Tag.
Séquence
Gln50-Ser177
Pureté
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human GITR Ligand Trimer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail.
TNFSF18
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
TNFSF18
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 90 % by SDS - PAGE
SDS
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Stock
4 °C,-80 °C
Stockage commentaire
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Date de péremption
12 months
Antigène
TNFSF18
(Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))
Gitrl Protein, AITRL Protein, GITRL Protein, TL6 Protein, hGITRL Protein, TNLG2A Protein, tumor necrosis factor (ligand) superfamily, member 18 Protein, TNF superfamily member 18 Protein, Tnfsf18 Protein, TNFSF18 Protein
Sujet
TNFSF18, AITRL, TL6, GITRL, GITR Ligand,Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.
Poids moléculaire
47.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.