TNFSF15
(Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))
Type de proteíne
Recombinant
Attributs du protein
AA 61-252
Origine
Souris
Source
HEK-293 Cells
Purification/Conjugué
Cette TNFSF15 protéine est marqué à la His-Avi Tag,Biotin.
Fonction
Biotinylated Mouse TNFSF15 Protein
Séquence
Ala61-Leu252
Attributs du produit
Recombinant Biotinylated Mouse TNFSF15 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala61-Leu252.
Pureté
> 90 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-TNFSF15 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 20.5ng/ml determined by ELISA. See testing image for detail.
TNFSF15
Origine: Souris
Hôte: Mammalian Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
TNFSF15
Origine: Cynomolgus
Hôte: Mammalian Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
TNFSF15
(Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))
TTPAL Protein, TL1 Protein, TL1A Protein, VEGI Protein, VEGI192A Protein, Tl1 Protein, Tl1a Protein, Vegi Protein, tumor necrosis factor superfamily member 15 Protein, TNF superfamily member 15 Protein, tumor necrosis factor (ligand) superfamily, member 15 Protein, TNFSF15 Protein, Tnfsf15 Protein
Sujet
TL1A and its functional receptor DR3 are members of the TNF/TNFR superfamilies of proteins.TL1A and DR3 are abundantly localized at inflamed intestinal areas of patients with IBD and mice with experimental ileitis or colitis and actively participate in the immunological pathways that underlie mucosal homeostasis and intestinal inflammation.Recently, an important role was demonstrated for TL1A/DR3 as potential mediators of intestinal fibrosis that is associated with the presence of gut inflammation.
Poids moléculaire
24.40 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Tris-Bis PAGE result.