GARP & Latent TGF beta 1 Complex (Tyr137His-Mutant) protein (His-Avi Tag)

N° du produit ABIN7274702

Détail du produit

Antigène: GARP & Latent TGF beta 1 Complex

Type de proteíne: Recombinant

Attributs du protein: Tyr137His-Mutant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: His-Avi Tag

Fonction: Human GARP (Y137H) & Latent TGF Beta 1 Complex Protein

Séquence: His20-Leu628 (GARP (Y137H) ) & Leu30-Ser390 (Latent TGF Beta 1)

Specificité: Uni-Prot: Q14392 (GARP (Y137H)), P01137 (Latent TGF beta 1)

Attributs du produit: Recombinant Human GARP(Y137H) & Latent TGF Beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His20-Leu628 (GARP(Y137H)) & Leu30-Ser390 (Latent TGF Beta 1).

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GARP (Y137H) &Latent TGF Beta 1 Complex, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-GARP&Latent TGF Beta 1 Antibody, hFc Tag with the EC50 of 24.8ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: GARP & Latent TGF beta 1 Complex

Sujet: GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.

Poids moléculaire: 70.3 kDa (GARP(Y137H))&41.4 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 71-75 kDa (GARP(Y137H))&13 kDa&42-45 kDa (Latent TGF Beta 1) based on Tris-Bis PAGE result.

UniProt: Q14392