IFNA Protein (AA 28-436) (His-Avi Tag)

N° du produit ABIN7274849

Protéine Recombinant IFNA exprimée dans HEK-293 Cells.

Aperçu rapide pour IFNA Protein (AA 28-436) (His-Avi Tag) (ABIN7274849)

Antigène: IFNA (Interferon alpha (IFNA))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 28-436

Purification/Conjugué: Cette IFNA protéine est marqué à la His-Avi Tag.

Fonction: Non-biotinylated Human IFN alpha/beta R1 Protein

Séquence: Lys28-Lys436

Attributs du produit: Recombinant Human IFN alpha/beta R1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys28-Lys436.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).,Immobilized Human IFN alpha/beta R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IFN alpha/beta R1 Antibody, hFc Tag with the EC50 of 16.0ng/ml determined by ELISA (QC Test).,Human IFN alpha/beta R1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of-Human IFN alpha/beta R1 is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IFNA (Interferon alpha (IFNA))

Autre désignation: IFN alpha

Sujet: IFN-alpha / beta R1, also known as IFNAR1, belongs to the class II cytokine receptor family of proteins. Class II cytokine receptors form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions.Functions in general as heterodimer with IFNAR2. Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves.

Poids moléculaire: 50.2 kDa. Due to glycosylation, the protein migrates to 74-95 kDa based on Bis-Tris PAGE result.

UniProt: P17181

Pathways: Signalistation JAK/STAT, Signalisation TLR, Hepatitis C, Inflammasome