IgE Protein (AA 209-429) (His-Avi Tag)

N° du produit ABIN7274854

Cette protéine Recombinant IgE est exprimée dans HEK-293 Cells.

Aperçu rapide pour IgE Protein (AA 209-429) (His-Avi Tag) (ABIN7274854)

Antigène: IgE

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Cynomolgus

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 209-429

Purification/Conjugué: Cette IgE protéine est marqué à la His-Avi Tag.

Fonction: Non-biotinylated Cynomolgus IgE Protein

Séquence: Cys209-Lys429

Attributs du produit: Recombinant Cynomolgus IgE Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Cys209-Lys429.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus IgE, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Omalizumab, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA.,Cynomolgus IgE on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus IgE is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IgE

Sujet: Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.

Poids moléculaire: 27.9 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.

UniProt: G8F4W7