IgE Protein (AA 198-421) (His-Avi Tag)
Aperçu rapide pour IgE Protein (AA 198-421) (His-Avi Tag) (ABIN7274857)
Antigène
Voir toutes IgE ProtéinesType de proteíne
Activité biologique
Origine
Source
Pureté
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Attributs du protein
- AA 198-421
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Purification/Conjugué
- Cette IgE protéine est marqué à la His-Avi Tag.
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Fonction
- Non-biotinylated Mouse IgE Protein
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Séquence
- Asp198-Ser421
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Attributs du produit
- Recombinant Mouse IgE Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp198-Ser421.
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Stérilité
- 0.22 μm filtered
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niveau d'endotoxine
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Mouse IgE on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse IgE is greater than 95% as determined by SEC-HPLC.
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IgE (AA 209-428) protein (His-Avi Tag)
Origine: Humain Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 209-429) (Active) protein (His-Avi Tag)Origine: Cynomolgus Hôte: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 91-421) (Active) protein (His tag)Origine: Souris Hôte: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE protein (Biotin)Origine: Humain Hôte: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, Func, FACS
IgE (AA 1-429) protein (His tag)Origine: Rat Hôte: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, Imm, PC
IgE (AA 1-428) protein (His tag)Origine: Humain Hôte: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
IgE (AA 1-432) protein (His tag)Origine: Cynomolgus Hôte: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Stock
- -20 °C,-80 °C
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Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Date de péremption
- 12 months
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- IgE
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Sujet
- Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.
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Poids moléculaire
- 28.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
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UniProt
- P06336
Antigène
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