IL17 Receptor B (IL17RB)
(Interleukin 17 Receptor B (IL17RB))
Type de proteíne
Recombinant
Attributs du protein
AA 18-292
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette IL17 Receptor B protéine est marqué à la His tag.
Fonction
Human IL-17RB Protein
Séquence
Arg18-Per292
Attributs du produit
Recombinant Human IL-17RB Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg18-Per292.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IL-17RB, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-17RB Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. The affinity constant of 0.81 nM as determined in SPR assay (Biacore T200). See testing image for detail.
IL17RB
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
> 85 % as determined by reducing SDS-PAGE.
Active
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
IL17 Receptor B (IL17RB)
(Interleukin 17 Receptor B (IL17RB))
Among inflammatory mediators, a growing body of evidence emphasizes the contribution of the interleukin 17 (IL-17) cytokine family in malignant diseases. Besides IL-17A, the prototypic member of the IL-17 family, several experimental findings strongly support the role of the IL-17B/IL-17 receptor B (IL-17RB) pathway in tumorigenesis and resistance to anticancer therapies. In mouse models, IL-17B signaling through IL-17RB directly promotes cancer cell survival, proliferation, and migration, and induces resistance to conventional chemotherapeutic agents.
Poids moléculaire
31.4 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.