IL-10 Protein (AA 19-178) (His-Avi Tag)

N° du produit ABIN7274896

Cette protéine Recombinant IL-10 est exprimée dans HEK-293 Cells.

Aperçu rapide pour IL-10 Protein (AA 19-178) (His-Avi Tag) (ABIN7274896)

Antigène: IL-10 (IL10) (Interleukin 10 (IL10))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 19-178

Purification/Conjugué: Cette IL-10 protéine est marqué à la His-Avi Tag.

Fonction: Non-biotinylated Human IL-10 Protein

Séquence: Ser19-Asn178

Attributs du produit: Recombinant Human IL-10 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ser19-Asn178.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IL-10R alpha, His Tag immobilized on CM5 Chip can bind Human IL-10, His Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-10, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.7ng/ml determined by ELISA (QC Test).,Human IL-10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human IL-10 is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-10 (IL10) (Interleukin 10 (IL10))

Autre désignation: IL-10

Sujet: Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.

Poids moléculaire: 21.3 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Bis-Tris PAGE result.

UniProt: P22301

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints