IL12 Protein (His-Avi Tag)

N° du produit ABIN7274898

Détail du produit

Antigène: IL12 (Interleukin 12 (IL12))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette IL12 protéine est marqué à la His-Avi Tag.

Séquence: Ile23-Ser328(IL-12B)/Arg23-Ser219(IL-12A)

Pureté: > 95% as determined by Tris-Bis PAGE,> 95% as determined by SEC-HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-12, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Stock: 4 °C,-80 °C

Stockage commentaire: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IL12 (Interleukin 12 (IL12))

Autre désignation: IL-12 (IL12 Produits)

Synonymes: CLMF Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12 Protein, LOC100217488 Protein, IL-12p35 Protein, Il-12a Protein, Ll12a Protein, p35 Protein, IL12p40 Protein, IL12B Protein, IL-12 p35 Protein, interleukin 12B Protein, interleukin 12A Protein, interleukin 12b Protein, interleukin 12a Protein, Interleukin 12 level Protein, IL12B Protein, IL12A Protein, Il12b Protein, Il12a Protein, IL12 Protein, il12a Protein

Sujet: CLMF, CLMF2, IL-12A, IL-12B, IL12, IL12 p70, IMD28, IMD29, NFSK, NKSF1, NKSF2, P35, IL-12 subunit p35, IL12A, interleukin 12,Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.

Poids moléculaire: 34.7(IL-12B)/25.5 (IL-12A) kDa. Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result.

Pathways: Signalistation JAK/STAT, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome