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IL-7 Protein (AA 26-177)

IL7 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
N° du produit ABIN7274963
  • Antigène Voir toutes IL-7 (IL7) Protéines
    IL-7 (IL7) (Interleukin 7 (IL7))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 26-177
    Origine
    • 19
    • 10
    • 5
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 17
    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Fonction
    Recombinant Human IL-7 Protein is expressed from E.coli without tag.It contains Asp26-His177.
    Séquence
    Asp26-His177
    Pureté
    > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human IL-7 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA (QC tested). The affinity constant of 7.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    IL-7 (IL7) (Interleukin 7 (IL7))
    Autre désignation
    IL-7 (IL7 Produits)
    Synonymes
    IL7 Protein, IL-7 Protein, A630026I06Rik Protein, Il-7 Protein, hlb368 Protein, interleukin 7 Protein, IL7 Protein, Il7 Protein
    Sujet
    IL7, Interleukin-7, IL-7, Lymphopoietin-1, PBGF,Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor.
    Poids moléculaire
    17.5 kDa same as Tris-Bis PAGE result.
    UniProt
    P13232
    Pathways
    Signalistation JAK/STAT
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