Cette IL22 Receptor alpha 1 protéine est marqué à la Fc Tag.
Fonction
Mouse IL-22R alpha 1 Protein
Séquence
His16-Ala228
Attributs du produit
Recombinant Mouse IL-22R alpha 1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains His16-Ala228.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Mouse IL-22R alpha 1, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse IL-22, His Tag with the EC50 of 0.23μg/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Interleukin (IL)-22 is a member of the IL-10 family of cytokines and represents an important effector molecule of activated Th22, Th1, and Th17 cells, as well as Tc-cell subsets, gammadelta T cells, natural killer (NK), and NKT cells. IL-22 mediates its effects via a heterodimeric transmembrane receptor complex consisting of IL-22R1 and IL-10R2 and subsequent Janus kinase-signal transducers and activators of transcription (JAK-STAT) signaling pathways including Jak1, Tyk2, and STAT3.
Poids moléculaire
51.1 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result.