Klotho beta Protein (KLB) (AA 30-983) (His tag)

N° du produit ABIN7275092

Protéine Recombinant Klotho beta exprimée dans HEK-293 Cells.

Aperçu rapide pour Klotho beta Protein (KLB) (AA 30-983) (His tag) (ABIN7275092)

Antigène: Klotho beta (KLB)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 30-983

Purification/Conjugué: Cette Klotho beta protéine est marqué à la His tag.

Fonction: Human Beta Klotho Protein

Séquence: Met30-Thr983

Attributs du produit: Recombinant Human Beta Klotho Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met30-Thr983.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200).,Immobilized Human Beta Klotho at 2ug/ml (100ul/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA (QC Test).,Immobilized Human Beta Klotho at 2ug/ml (100ul/Well). Dose response curve for Anti-Beta Klotho Antibody2, hFc Tag with the EC50 of 34.6ng/ml determined by ELISA (QC Test).,Immobilized Human FGF21, mFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.75ug/ml determined by ELISA.,Human Beta Klotho on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Human Beta Klotho is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Klotho beta (KLB)

Autre désignation: Beta Klotho

Sujet: Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites.

Poids moléculaire: 111.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result.

UniProt: Q86Z14

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Growth Factor Binding