TNFSF14
(Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
Type de proteíne
Recombinant
Attributs du protein
AA 74-240
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette TNFSF14 protéine est marqué à la His tag.
Fonction
Human LIGHT/TNFSF14 Protein
Séquence
Asp74-Val240
Attributs du produit
Recombinant Human LIGHT/TNFSF14 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Asp74-Val240.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human LIGHT, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail.
TNFSF14
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
Active
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
TNFSF14
(Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.
Poids moléculaire
19 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.