LILRB2
(Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2))
Type de proteíne
Recombinant
Attributs du protein
AA 22-458
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette LILRB2 protéine est marqué à la His-Avi Tag.
Fonction
Human LILRB2/CD85d/ILT4 Protein
Séquence
Gln22-His458
Attributs du produit
Recombinant Human LILRB2/CD85d/ILT4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln22-His458.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human LILRB2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. See testing image for detail.
LILRB2
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 97 %
WB, SDS, Imm, PC
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
LILRB2
(Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2))
CD85D Protein, ILT-4 Protein, ILT4 Protein, LIR-2 Protein, LIR2 Protein, MIR-10 Protein, MIR10 Protein, leukocyte immunoglobulin-like receptor subfamily A member 3 Protein, leukocyte immunoglobulin like receptor B2 Protein, LOC100433990 Protein, LILRB2 Protein
Sujet
The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles.
Poids moléculaire
50.2 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Tris-Bis PAGE result.