Lrig1
(Leucine-Rich Repeats and Immunoglobulin-Like Domains 1 (Lrig1))
Type de proteíne
Recombinant
Attributs du protein
AA 35-779
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette Lrig1 protéine est marqué à la His tag.
Fonction
Human LRIG1 Protein
Séquence
Ala35-Ser779
Attributs du produit
Recombinant Human LRIG1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala35-Ser779.
Pureté
> 95 % as determined by Tris-Bis PAGE
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human LRIG1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-LRIG1 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA. See testing image for detail.
Lrig1
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 50 mM MES, 150 mM NaCl, 1 mM EDTA ( pH 5.0).
Buffer
Lyophilized from 0.22μm filtered solution in 50 mM MES, 150 mM NaCl, 1 mM EDTA ( pH 5.0). Normally 8 % trehalose is added as protectant before lyophilization.
Agent conservateur
Other preservative
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
Lrig1
(Leucine-Rich Repeats and Immunoglobulin-Like Domains 1 (Lrig1))
LIG-1 Protein, LIG1 Protein, D6Bwg0781e Protein, Img Protein, leucine rich repeats and immunoglobulin like domains 1 Protein, leucine-rich repeats and immunoglobulin-like domains 1 Protein, LRIG1 Protein, Lrig1 Protein
Sujet
The leucine-rich repeats and immunoglobulin-like domains (LRIG)-1 is a tumor suppressor gene that belongs to the LRIG family. LRIG1 expression has prognostic significance in various human cancers. Somatic mutations, which are associated with a certain rate of response to targeted therapies, are ubiquitously found in human non-small cell lung cancer (NSCLC). LRIG1 was an independent prognostic factor for OS of NSCLC patients. LRIG1 in combination with other clinicopathological risk factors was a stronger prognostic model than clinicopathological risk factors alone.
Poids moléculaire
83 kDa. Due to glycosylation, the protein migrates to 85-105 kDa based on Tris-Bis PAGE result.