Mesothelin Protein (MSLN) (His tag)

N° du produit ABIN7275280

Détail du produit

Antigène: Mesothelin (MSLN)

Type de proteíne: Recombinant

Origine: Cynomolgus

Source: HEK-293 Cells

Purification/Conjugué: Cette Mesothelin protéine est marqué à la His tag.

Séquence: Asp296-Gly580

Pureté: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus MSLN at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MSLN Ab., hFc Tag with the EC50 of 14.6ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Stock: 4 °C,-80 °C

Stockage commentaire: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Mesothelin (MSLN)

Autre désignation: MSLN (MSLN Produits)

Sujet: MSLN, CAK1 antigen, CAK1, megakaryocyte potentiating factor, Mesothelin, MPF, MPFSMRP,Pre-pro-megakaryocyte-potentiating factor, SMR, soluble MPF mesothelin related protein,Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31- kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

Poids moléculaire: 33 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result.

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process