KLRK1
(Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))
Type de proteíne
Recombinant
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette KLRK1 protéine est marqué à la His-Avi Tag,Biotin.
Séquence
Phe78-Val216
Pureté
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Biotinylated Human NKG2D, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
KLRK1
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
ELISA
KLRK1
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
>90 % as determined by reduced SDS-PAGE.
Active
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Stock
4 °C,-80 °C
Stockage commentaire
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Date de péremption
12 months
Antigène
KLRK1
(Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))
CD314, D12S2489E, KLR, NKG2-D, NKG2D,NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
Poids moléculaire
19.0 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Tris-Bis PAGE result.