Recombinant Human NKp46/NCR1/CD335 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln22-Asn254.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human NKp46, His Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-NKp46 Ab., hFc Tag with the EC50 of 9ng/ml determined by ELISA. See testing image for detail.
NCR1
Origine: Rat
Hôte: Escherichia coli (E. coli)
Recombinant
> 90 %
SDS, WB, Imm, PC
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR).These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells.
Poids moléculaire
27.5 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.