Cette Oncostatin M Receptor protéine est marqué à la His tag.
Fonction
Human OSMR Protein
Séquence
Glu28-Met740
Attributs du produit
Recombinant Human OSMR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu28-Met740.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human OSMR, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
OSMR
Origine: Rat
Hôte: Escherichia coli (E. coli)
Recombinant
> 95 %
WB, SDS, Imm, PC
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
OSMRB Protein, PLCA1 Protein, oncostatin M receptor Protein, OSMR Protein, Osmr Protein
Sujet
OSMR is targeted to the mitochondrial matrix via the presequence translocase-associated motor complex components, mtHSP70 and TIM44. OSMR interacts with NADH ubiquinone oxidoreductase 1/2 (NDUFS1/2) of complex I and promotes mitochondrial respiration. Deletion of OSMR impairs spare respiratory capacity, increases reactive oxygen species, and sensitizes BTSCs to IR-induced cell death.
Poids moléculaire
82.2 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result.