Recombinant Cynomolgus PSMA/FOLH1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Lys44-Ala750.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus PSMA, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 37.5ng/ml determined by ELISA. See testing image for detail.
FOLH1
Origine: Souris
Hôte: Mammalian Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
FOLH1
Origine: Cynomolgus
Hôte: Mammalian Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
FOLH1
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
Restrictions
For Research Use only
Format
Liquid
Buffer
Supplied as 0.22μm filtered solution in PBS ( pH 7.4).
Stock
-80 °C
Stockage commentaire
Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.
Poids moléculaire
80.6 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.