RSPO1 Protein (AA 31-263) (His tag)

N° du produit ABIN7275537

Cette protéine Recombinant RSPO1 est produite dans CHO Cells.

Aperçu rapide pour RSPO1 Protein (AA 31-263) (His tag) (ABIN7275537)

Antigène: RSPO1 (R-Spondin 1 (RSPO1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: CHO Cells

Pureté: > 95 % as determined by Bis-Tris PAGE

Détail du produit

Attributs du protein: AA 31-263

Purification/Conjugué: Cette RSPO1 protéine est marqué à la His tag.

Fonction: Human R spondin 1/RSPO1 Protein

Séquence: Arg31-Ala263

Attributs du produit: Recombinant Human R spondin 1/RSPO1 Protein is expressed from CHO with His tag at the C-Terminus.It contains Arg31-Ala263.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 0.05 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human R spondin 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).,Immobilized Human R spondin 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4ug/ml determined by ELISA.,Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human R spondin 1, His Tag with the EC50 of 4.4ug/ml determined by ELISA.,Human R spondin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50-for this effect is 1.0 - 10.0 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test).,Loaded Human R spondin 1, His Tag on Anti-His-Biosensor can bind Human RNF43, hFc Tag with an affinity constant of 18.50 nM as determined in BLI assay (Gator® Prime).,Loaded Human R spondin 1, His Tag on Anti-His-Biosensor can bind Human LRP-6, mFc Tag with an affinity constant of 8.97 nM as determined in BLI assay (Gator® Prime).

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: RSPO1 (R-Spondin 1 (RSPO1))

Autre désignation: R spondin 1

Sujet: The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.

Poids moléculaire: 27.3 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result.

UniProt: Q2MKA7